Cytology and Meristems Vocabulary

Cell types
Collenchyma: elongate cells with thickened primary wall facing intercellular spaces, having immature plastids, but otherwise like parenchyma
Meristematic: undergoing cell divisions, typically small and thin-walled, lacking mature plastids or vacuoles.
Parenchyma: thin-walled, complex cytoplasm, active biochemistry, sometimes isodiametric, sometimes with large intercellular spaces
Sclerenchyma: cells with heavy, lignified secondary walls, usually dead at maturity, protoplast missing.
Cell structures
Amyloplast: a colorless plastid consisting mostly of starch (amylose)
Cell membrane=
plasma membrane:
a phospholipid bilayer including intrinsic and extrinsic proteins for facilitated diffusion and active transport
Chloroplast: a mature plastid developing grana stacks of thylakoid membranes holding chlorophyll; responsible for photosynthesis
Chromoplast: a plastid with membranes holding primarily yellow or orange carotenoid pigments instead of chlorophyll
Cristae: inner membrane of mitochondrion folded and specialized for oxidative phosphorylation and the electron transport system
Cytoplasm: all fluids and organelles of the cell found inside the cell membrane, but usually not including the nucleus
Cytosol: the fluid surrounding the organelles consisting of water and dissolved solutes, notably enzymes, substrates, and products; responsible for glycolysis and fermentation
Endoplasmic reticulum: the endomembrane system responsible for movement of materials from one area of a cell to another, sometimes for membrane synthesis, sometimes studded with ribosomes on its cytosolic face
Golgi apparatus=
dictyosome:
a stack of endoplasmic reticulum membranes responsible for sorting and packaging cell products for export via vesicles
Leucoplast: a colorless plastid
Matrix: the fluid inside the mitochondrion, containing 70S ribosomes; naked, circular DNA; and responsible for the Krebs cycle (Citric Acid cycle, TCA cycle)
Microfilament: a small-diameter fiber of the cytoskeleton composed of actin; responsible for certain types of cytoplasmic movements
Microsome=
microbody:
small organelles of several types, including lysosomes, peroxysomes, glyoxysomes, etc. responsible for certain metabolic pathways or portions thereof
Microtubule: a large-diameter fiber of the cytoskeleton composed of tubulin; responsible for chromosome movement during mitosis and meiosis, also involved with flagellar movement
Middle lamella: a pectinaceous glue holding cells together
Mitochondrion: organelle responsible for the Krebs cycle (Citric Acid cycle, TCA cycle), and Oxidative phosphorylation reactions of respiration
Nuclear envelope: the bounding membrane of the nucleus, derived from and continuous with the endoplasmic reticulum
Nucleolus: a dark-staining body inside the nucleus, believed to be the site of ribosomal RNA synthesis and ribosome-subunit assembly
Nucleus: an organelle containing the genomic DNA for the cell, responsible for transcription and replication; involved in mitosis
Plasmodesma: a cytoplasmic connection penetrating both walls of adjacent cells, probably the result of incomplete wall formation from a phragmoplast during cytokinesis
Plastid: a class of organelles including chloroplasts, chromoplast, leucoplasts, amyloplasts, and proplastids
Primary cell wall: a multi-layered containment for the cell consisting of cellulose fibers, cross-linked by hemicellulose and other polysaccharides, also including protein.
Proplastid: the archetype form of plastids, can develop into one of several types of plastids
Protoplast: a plant cell lacking a cell wall; the cell membrane and its contents
Ribosomes: 80S particles consisting of RNA and protein, responsible for protein synthesis (translation); sometimes free-floating, sometimes associated with the endoplasmic reticulum
Secondary wall: an additional wall produced inside the primary wall, usually in three layers, consisting of cellulose, cross-linking polysaccharides, and up to 50% lignin polymers
Stroma: the fluid inside the chloroplast, containing 70S ribosomes; naked, circular DNA; and responsible for the carbon-fixation reactions (Calvin cycle) of photosynthesis
Thylakoid membrane: a membrane system, usually forming grana stacks, and holding chlorophyll by means of intrinsic membrane proteins: responsible for the light reactions of photosynthesis
Tonoplast: the vacuole membrane
Vacuole: a fluid compartment inside plant cells bounded by the tonoplast; responsible for toxic waste processing and storage
Vesicles: small membrane bound packages containing materials for import or export
Root tip
Columella: cells in the middle of the root cap responsible for gravity perception possibly by means of starch statoliths
Ground meristem: a primary tissue maturing to become cortex
Protoderm: a primary tissue maturing to become the dermal system
Provascular tissue=
procambium:
a primary tissue maturing to become the vascular cylinder
Quiescent center: a group of pseudo-meristematic cells just under the root cap which are perhaps the source of cells recruited into the meristem
Root apex: meristem plus associated root cap
Root cap: meristematic and parenchymatous layer over end of root tip, sloughing cells at the periphery, secreting mucilage
Root hair: an extension of a single epidermal hair, increasing surface area and secreting protons to release mineral cations from clay particles
Root meristem: a zone of meristematic cells just proximal to the quiescent center, responsible for adding cells to the length of the root.
Zone of cell division: areas of mitotic division including the root meristem and the root cap
Zone of cell elongation: areas of rapid cell elongation usually just proximal to the zone of cell division
Zone of cell maturation: areas in which cells are maturing into xylem, phloem, root hairs, etc.; usually just proximal to the zone of cell division
Shoot Tip
Apical bud: a the shoot apex consisting of the shoot meristem and its associated leaf primordia
Axillary bud=
lateral bud:
a branch apex developing in the narrow angle between the stem and leaf primordium; may be dormant or active
Internode: the portion of the stem between nodes; cell division (interstitial or intercalary) and cell elongation are likely to occur here to extend the shoot tip and increase the height of the plant
Leaf primordium: an immature leaf
Node: a layer across the stem at the level of leaf attachment; neither cell division nor cell elongation is not likely to occur
Primary tissues: protoderm, ground meristem, provascular tissues (see under root tip above)
Shoot meristem=
apical dome:
meristematic cells at tip of stem axis
Zones of cell division,
elongation,
and maturation:
in stems these areas are dispersed throughout the developing stem, rather than distributed sequentially along the length as in roots (see under Root tip, above)
Growth Forms and Directions
Abaxial: the surface facing away from the stem; in a leaf the lower epidermis
Acropetal: developing upward, toward the apex
Adaxial: the surface facing the stem; in a leaf, the upper epidermis
Apical dominance: the apical bud producing hormones which keep the lateral (axillary) buds dormant
Apical growth: increase in length by addition of new cells at the tip of a leaf primordium (to form petiole and midrib)
Apical: near the apex
Basal: near the surface of the soil
Basipetal: developing downward, toward the base
Centrifugal: developing from the inside outward (sometimes from apex downwards)
Centripetal: developing from the outside inward (sometimes from bottom upwards)
Determinate growth: growth which results in a "final" size that does not change for the remaining life of the organ; typical of many dicot leaves and certain stems
Indeterminate growth: growth which continues indefinitely during the life of the plant; typical of certain stems and certain kinds of monocot leaves.
Intercalary growth: increase in cell size and cell number in basal region of internode or leaf blade (as in grasses).
Interstitial growth: increase in cell size and cell number throughout small, but fully formed leaf primordium to produce mature leaf size
Marginal growth: increase in width by addition of new cells along the edges of a leaf primordium (to form blade)
Cell Division/Cell Cycle
Anaphase: the third portion of mitosis in which the centromere dissociates and the microtubules from each end depolymerize to pull sister chromatids to opposite ends of the cell
Cytokinesis: the division of the cytoplasm of a dividing cell to form two new cells; in plants accomplished by formation of a phragmoplast at the equator
G1: the first gap of interphase in which a cell which has just completed division carries out normal biochemsitry and prepares for synthesis of DNA
G2: the second gap of interphase in which the cell has completed replication of its DNA and is preparing for mitosis and cytokinesis; normal biochemistry occurs
Interphase: a long portion of the cell cycle in which DNA is decondensed as chromatin, consisting of three portions: G1, S, G2
Metaphase: the second portion of mitosis in which the chromosomes are pushed to the equator of the cell by spindle fibers (microtubules) which elongate from the poles and attach to both sides of the centromere of each chromosome
Mitosis: nuclear division consisting of prophase, metaphase, anaphase, and telophase; a diploid (2N, 4C) cell divides its chromatids into separate diploid nuclei (2N 2C)
Prophase: the first portion of mitosis in which the chromosomes condense out of the chromatin, the nuclear envelope disappears, and the nucleolus disappears
S: the synthesis portion of interphase during which the DNA of the nucleus is replicated; a diploid cell (2N) becomes 4C, a haploid cell (1N) becomes 2C; the copies are held together at a region eventually becoming the centromere
Telophase: the final portion of mitosis in which the nuclear envelope reforms, the chromosomes decondense to form chromatin, and the nucleolus reappears



This page © Ross E. Koning 1994.

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