Biology 332
Spring 1994
Exam IName___________________________

Reasons to study plants. While you are taking this exam, Dr. Koning will be making a recipe. The recipe is shown below. For each ingredient or step outlined below, tell how each involves plants.
In large mixing bowl cream together:

1 C Margarine

1.5 C Sugar

1T Molasses

1 t Vanilla

2 Eggs

1 t baking soda

1 t salt

Fold in the following, scraping the sides of the bowl frequently

2.25 C flour

Add 12 oz package of Chocolate Chips.

Drop by teaspoons onto aluminum baking sheet.

Bake 9-11 minutes at 350 F (178 C).

Clean and dry the dishes

Store cooled cookies in Tupperware container

On this page, draw a parenchyma cell (15 cm diameter!) showing all major organelles. Label your diagram completely with the names of structures. Next to each name label, indicate the basic function of each structure.

Examine the graph below showing the rates of respiration and photosynthesis for a particular plant over a range of light intensities:

Which line represents photosynthesis?

What is happening to the plant at 100 fc?

What is happening to the plant at 1000 fc?

What is happening to the plant at 30,000 fc?

What is causing line A to curve upward between 0 and 1000 fc?

Why does line A "level off" between 1000 and 30,0000 fc?

What is the name for the intensity of light where A and B cross?

Which light intensity shows the fastest rate in curve A?

Why is curve B so straight (flat)?

Which curve most likely represents the Hill Reaction (photolysis)?

Which curve most likely represents the Calvin Cycle?

Provide the overall reaction for photosyntheis:




Circle the components involved with the light reactions.
Put a square around components involving the Calvin Cycle.

List the steps of mitosis in order. Tell what happens in each phase.
Name of Phase (in order)What happens in this phase

Explain the fundamental differences between the first division of meiosis and mitosis.






You work in a biotechnology company, producing some interesting flavorings from cell cultures of Mentha spicata (spearmint). A cell culture is a liquid medium with cells floating in it. Your job is to increase the production of the flavoring chemical.

What cell type will you need to have in mass culture to produce the flavor?_____________

What cell type will you need to cultivate to get the needed cell type?__________________

What living cell type would be required in a living mint plant
but is NOT needed in your cell culture
(in fact its development would limit flavor yield)?______________________________

In growing your cell cultures, you notice that older cultures have some wierd cells. These are nearly spherical, like most of the others, but the cells contain a worm-like spiral of material.

On which side of the original cell wall will you find the spiral?_____________________

Do you expect the spiral to be inside the cell membrane or outside?__________________

Will the spiral pick up Safranin (a red dye) or Fast green (a green dye)?_______________

By what cell type would you classify these wierd cells?___________________________

Attempts to culture these cells alone fail, why?__________________________________

Compare and contrast the composition, cell structure and function of xylem and phloem.
Xylem Tissue CompositionPhloem Tissue Composition


Xylem Tissue Conductive Cell StructurePhloem Conductive Cell Structure


Xylem FunctionPhloem Function



Suzy Homemaker is making some tea for a tea party. The tea kettle has a whistling spout cover that spurts a stream of steam and whistles as the water boils. Suzy puts the kettle on but gets busy with her arriving guests. The kettle whistles merrily away. Suzy has a plant near the stove and the stream of steam shoots all around the stem at a point just above the soil. The steam is hot enough to kill any cell in its path. There is no immediate indication of what happened, but what WILL happen to the plant after Suzy's party is over? Be sure to think of the WHOLE plant!






Briefly describe what advantage is conferred by the evolution of C-4 photosynthesis.






How does CAM photosynthesis differ from C-4 photosynthesis, and why does this offer any advantage to a plant?






Distinguish between a simple and a compound leaf.

A simple leaf___________________________________________________

A compound leaf________________________________________________

I have a plant discovered in a tropical jungle. How can I tell whether it is a dicot or a monocot? Hint: there are two clear ways you already know about from lab.
1. _________________________________________________________________

2. _________________________________________________________________

Dr. Koning decided against using a phosphate-citrate buffer and instead chose a potassium-phosphate buffer in the photosynthesis exercise (floating leaf discs). Why would he try to avoid citrate in a lab about photosynthesis? How could it have interfered with the project?






Looking back at page 3 you notice a photosynthesis graph. How could we generate a similar graph in the laboratory using our syringes and leaf discs?






What would we have to do to generate the respiration graph?






What other characteristic of light has not been tested in lab, and how might you do so with the syringes and leaf discs?






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