Plant Physiology

Tentative Schedule

Biology 438 Fall 2015
MWF 9-9:50 PM Lecture Science 432
T 11:00 AM-1:50 PM Laboratory Science 318
Hotlines: 860-465-4444 or 800-578-1449

Dr. Ross Koning
MWF 10-11, MF 11-12N Science 356
Cell: 860-933-2712

Date Lecture/Lab Topic/Activity/Etc.
Mon-Aug 31 Introduction: Science
Tue-Sep 1 No Quiz — Statistics
F15 Leaf Data
Wed-Sep 2 Why Study Plants?
Fri-Sep 4 Basic Statistics
Mon-Sep 7 Labor Day
Tue-Sep 8 Due: Statistics Worksheet
Quiz 1 — Morphology and Tissue Culture
Wed-Sep 9 Basic Botany
Fri-Sep 11 Basic Botany (continued)
Mon-Sep 14 More Basic Botany
Tue-Sep 15 Due: Plant Morphology Amp. Abs.
Quiz 2 — Plant Anatomy
Leaf Cross Section
Stem Cross Section
Root Cross Section
Wed-Sep 16 Finish Basic Botany
Fri-Sep 18 Finishing Up Basics
Mon-Sep 21 Basic Plant Cytology 1
Tue-Sep 22 Due: Plant Anatomy Amp. Abs.
Quiz 3 — Cytology
Sample Abstract
Wed-Sep 23 Basic Plant Cytology 2
Fri-Sep 25 Cytoskeleton, Cell Cycle
Mon-Sep 28 Enzyme Basics
Tue-Sep 29 Due: Plant Cytology Amp. Abs.
Quiz 4 — Enzyme Kinetics Epic Fail
SpectroVis Protocol
Data Share Document
Excel Solution
Michaelis-Menten Regression
Nonlinear Regression Pointers
Term Report Assignment
Michaelis-Menten Translators
Michaelis-Menten Translation
Klabunde et al. Article
Janovitz-Klapp et al. Article
Wed-Sep 30 Enzyme Kinetics
Fri-Oct 2 Mineral Requirements
Mon-Oct 5 Finish Minerals
Water and Water Movement
Tue-Oct 6 Quiz 5 —Enzyme Kinetics Repeat
for three groups
2 other groups: M&M Data Analysis
Wed-Oct 7 Finish Water Movement
Fri-Oct 9 Root intake
Mon-Oct 12 Transpiration
Tue Oct 13 Due: Figures for Term Paper
Quiz 6 — Diffusion
and Betacyanin Structure
Sow Radish replacements
Wed-Oct 14 Solutes
Fri-Oct 16 Finish Solutes
Mon-Oct 19 Translocation
Tue-Oct 20 Due: Osmosis Data+Spreadsheet
Quiz 7 — Mineral Nutrition
Periodic Chart
Treat: Tissue Culture
Wed-Oct 21 Finish: Translocation
Fri-Oct 23 Light and Chlorophyll
Date Lecture/Lab Topic/Activity/Etc.
Mon-Oct 26 Finish: Light and Chlorophyll
Tue-Oct 27 Due: Diffusion Amp. Abs.
Quiz 8 — Transpiration
Planting Seeds, etc.
Wed-Oct 28 Light Reactions
Fri-Oct 30 Finish: Light Reactions
Sunday: Plant Mung Beans for Root Init.
Mon-Nov 2 Intro: Calvin Cycle
Tue-Nov 3 Due: Transpiration Handout Only
Sunglasses Recommended (Class 2012)
Quiz 9 — Photosynthesis
Replenish Mineral Elements
Plant Peas for Light
Treat: Pea Stem Growth
Wed-Nov 4 Calvin Cycle
Fri-Nov 6 Due Noon: Enzyme lab report
Finish: Calvin Cycle REK: Mung Beans for Root Initiation
Mon-Nov 9 C4 and CAM Cycles
Tue-Nov 10 Due: Photosynthesis Amp. Abs.
Quiz 10 — Model System: Happy Bird
Finish: Pea Stem Growth
Plant: Bean Branch Initiation
Plant: Bean Leaf abscission
Horm/Gen Pea Stem Growth Plot
Wed-Nov 11 Pentose Phosphate Shunt
Plant Respiration
Fri-Nov 13 Lab Treat: Root Initiation
Mon-Nov 16 Finish Phytochrome
Tue-Nov 17 Due: Happy Bird Handout Only
Due: Pea Stem Growth Amp. Abs.
Quiz 11 — Finish: Plant Tissue Culture
Tissue Culture Plot
Measure: Mineral Nutrition
Measure: Light and Stem Growth
Plant "Hard Wheat Berries" Seed
Wed-Nov 18 Photoperiodism
Fri-Nov 20 Blue-Light Responses
Mon-Nov 23 Finish: Blue-Light Responses
Tue-Nov 24 Due: Tissue Culture Amp. Abs.
Quiz 12 — Finish: Mineral Nutrition (DW)
Finish: Light and Stem Growth (DW)
Light and Stem Analysis
Finish: Root Initiation
2015 Rooting Data & Plot
Treat: Bean Branch Initiation
Treat: Leaf Abscission
Wed-Nov 25 Thanksgiving
Fri-Nov 27
Mon-Nov 30 Auxins
Tues-Dec 1 Due: Rooting Team Data Sent Via Email (no abstract)
2015 Rooting Data & Plot
Due: Light and Growth Data Print (no abstract)
Light and Stem Analysis
No Quiz — Finish: Bean Branch Initiation (IAA teams)
Bean Branch: Auxin
Treat: Leaf Senescence
Senescence Plots
SetUp: Seed Germination
Tomato Juice: 0.533 osmolar and pH 4.2
Wed-Dec 2 More About Auxin
Fri-Dec 4 Finish Seed Germination Project
Tomato Juice Plot
pH Plot
Sucrose Plot
ABA Dose Plot
GA Dose Plot
Seed Germination Z-test Sheet
Mon-Dec 7 Gibberellins
Tue-Dec 8 Due: Mineral Nutrition Amp. Abs.
Due: Leaf Senescence Amp. Abs.
Quiz 14 — Finish: Leaf Abscission
Abscission Plots
Finish: Bean Branch Initiation (CK teams)
Bean Branch: Cytokinin
Team Presentations on Seed Germination
(No abstract needed and only one copy of graph needed to hand in)
Ross' Presentation on Mung Bean rooting
Wed-Dec 9 Ethylene, Abscisic Acid
TBA-Dec TBA Bio Club Study TBA time Science TBA
Tue-Dec 15 Due: Bean Branch Amp. Abs.
Leaf Abscission Amp. Abs.
11 AM - 1 PM Comprehensive Final Exam in Science 318

This course satisfies one of the six upper-level course requirements for the biology major. It is designed to provide you with comprehensive exposure to the subject of plant physiology. You will learn about the structure and function of plants throughout their development from seeds through reproduction. Considerable experience in chemistry is assumed, as is recall from BIO 220 (Cell Biology). Our discussions and exercises will cover from the biochemical level through the organismal level. The laboratory exercises will complement the lectures. If you are a person who has over-specialized in molecular biology, zoology or human biology, this course will expand your horizons significantly. As a study of producers, this course will examine those organisms so important because of their position at the energy and elemental intake portion of the energy pyramid and the food web! Upon these organisms depends human survival.

You will find lecture notes, lab exercises, due dates, and other course materials available for you on the World Wide Web at this address: There may be a required username:___________________ and password:___________________ to access some of these materials as they are copyrighted and therefore cannot be given out over the internet beyond the members of our class.

Several texts in Plant Physiology are published...I have chosen none of them because they are too deep (expecting too much background undergrads do not have yet) or are extremely expensive or both. If you want a finished, published text, older editions (except the first edition) are probably just fine for this course, but here are references for latest edition choices:

L. Taiz and E. Zeiger. 2015. Plant Physiology. 6th ed. Sinauer Associates, Inc. This is the deep, standard text at $145. Earlier editions might be just as useful for a whole lot less; just less "up to date."

R. Jones and H. Ougham. 2012. The Molecular Life of Plants. Wiley/Blackwell. About $100; I have not see this one yet, but it replaces a very good book that was more heavily biochemical.

W. G. Hopkins and N. P. A. Huner. 2009. Introduction to Plant Physiology. 4th ed. John Wiley & Sons, Inc. While more introductory than Taiz and Zeiger, it still costs $142. Go figure.

Separate exercise worksheets will be handed out for your use. You should obtain a three-ring binder to hold them together as a laboratory manual. The exercises will overlap in time and due-dates, so having them all together and with you in class each day is critical! The binder will help with that. I know spiral books are all the rage, but a 3-ring binder is vastly superior for this course! Put your name inside your binder so when it is lost you have at least some hope of recovery!

Large three-ring binder with zipper pouch containing: Small Scissors, Metric Rule, Fine Forceps, Pencil (mechanical preferred), Eraser, Simple Calculator (+−×÷=). Former students suggest getting some color pencils for your zipper pouch. A small pocket knife or whatever you like to clean under your fingernails would be good to have too. You absolutely need to have a USB=flash=jump drive. All of these are available inexpensively at retail stores just about anywhere. The alternative to the USB drive is to bring your own laptop with Microsoft Excel installed to some lab sessions (not inexpensive but maybe most useful to you)! Lab worksheets even partially completed in ink will receive a 10% penalty...use pencil only...keep it in your zipper pouch so it is ready for use!

Your final course grade will be based on weekly quizzes, laboratory worksheets and amplified abstracts, a lab report, and a final exam as described below.

There will be a quiz given each week for the first few minutes of laboratory time. Emphasis for each quiz will be whatever has been covered since the previous quiz in either lecture or laboratory, but questions could be on anything previously in the course and/or synthesis of separate ideas presented in the course. Each quiz may consist of a variety of question types; be prepared for all! You will be given one week after each quiz is returned in which to challenge, in writing, the grading/scoring of that quiz. THERE WILL BE NO MAKE-UP QUIZZES! Being tardy for the quiz will shorten your time to work on the quiz...when all students who are on-time to class are finished, all quizzes will be collected promptly. You be prompt too! Quizzes are collectively worth 35% of the course grade.

Each laboratory exercise will be inspired by a handout. You will work with one or more partner(s). Each person will fill out and hand in their own handout and/or write a one-page abstract about the project amplified by attached notes, drawings, graphs, calculations, etc., depending on the exercise. Electronic files are not acceptable documentation of your laboratory exercises. The worksheet and/or amplified abstract will be due one week after the exercise is officially completed and the due date will be posted on the official syllabus page for this course on the website. The papers are due at the beginning of the class period on the indicated date. Papers received after the starting time of the class by even 1 minute will be considered one-day late. Late papers will receive a penalty of 10% per day late but after the first graded exercise paper is returned to one of your classmates, your late submission is no longer acceptable and earns a grade of 0%! I grade as promptly as possible, so you need to be prompt too! The laboratory exercises are collectively worth 35% of the course grade.

The format of the lab report must follow the standard guidelines in the departmental style manual (Pechenik or Knisely). The laboratory report must be handed in as a hard-copy; electronic files are not acceptable. The laboratory report is due no later than 5 PM on Friday, October 30, 2015; failure to hand in this report by this deadline will result in a course grade of F, regardless of the other grades earned in the course. The laboratory report is worth 10% of the course grade. You should not let this assignment go to the deadline without any attention on your part! This needs to be an upper-level report showing your experience in our core writing courses.

You will take a comprehensive final exam in this course at the appropriate time for laboratory on the official final exam schedule. The exam covers all material in this course. Studying the quizzes will assist you in preparing for the final. But you will also want to be prepared to answer broader questions, perhaps even integrative essay questions that go beyond weekly coverage. The Final Exam constitutes 20% of the course grade.

Participation in this laboratory course is essential but, due to its subjective nature, is not given grading credit in a specific numerical sense. However, should your course grade come near a grading border, my sense of your participation in this course will be used as leverage into or barrier from the next-higher grade. Being on-time to all classes, having all materials needed for class, turning in assignments on time or early, being thorough in your laboratory work, being attentive in both lab and lecture classes, working efficiently and cooperatively with lab partners, asking pertinent questions, having answers to my questions in lectures, etc. are all good ways to impress me about your commitment to learning about plant physiology. If you are a person who procrastinates, who does only the minimum, who is tardy with everything, who complains about academic workloads, who watches lab partners doing the work, who sleeps in class, or who can not or will not do simple math, will get what you earn...and only what you earn.

Dress Code: This class will be consistent with departmental guidelines for laboratory courses. You are expected to wear closed shoes for all laboratory meetings. You are expected to wear long pants or jeans or will bring a labcoat to cover your shorts/dress/skirt. You will wear your slacks at your waist and will not be exposing the class to your undergarments. In laboratory, you will not wear shirts/blouses/sweaters/etc. with long flowing sleeves. You will not be allowed to wear a hat in lecture or laboratory, but if your hair is longer than your earlobes, you will be required to have your hair held back in a hair tie or elastic band, etc. for laboratory. You are not allowed to eat or drink in the laboratory.

If you are a commuter to our campus, you should remember that parking is often hard to find and leave extra time for the search, and for the walking that may be required once you are parked. Also, you need to think about alternatives should you have vehicle problems. Is there a family member who can give you a ride? Do you have contacts for other ECSU students from your town with whom you might carpool? Are your tires good enough for the driving conditions that New England weather will present? Commuters need to be courageous about driving in snow, on glare ice, in torrential rain, etc.

If you believe you will need special accommodations for this class, please contact the Office of AccessAbility Services at (860) 465-5573 as soon as possible. I cannot provide accommodations until I have received a formal accommodation letter from the Office of AccessAbility Services.

In many exercises you will work with laboratory partners and will share the data obtained. Your calculations, your reports, abstracts, and quizzes must, however, be done ON YOUR OWN. Plagiarism will not be tolerated and severe penalties will be invoked. Copying will not be tolerated. Extra credit work will not be given to any one for any reason!