This exercise is one of preparation to be used throughout the term and therefore will not be handed in at any time. Keep this in your notebook, and use it as directed by the instructor.
Biology is the study of life and, in order to study living organisms, we must grow them up to a suitable size for observation. At the beginning of the semester, we will do some mindless chores so that we will have growing plants to use in future exercises. Your instructor will demonstrate how to prepare soil and how to fill pots to receive plants or seeds to grow in the greenhouse. The instructor will also demonstrate how to put the pots onto the automated watering system in the greenhouse. It is your responsibility to be sure all of your pots are properly connected to the watering system! Be sure you know how to do this and check each new pot carefully before leaving the greenhouse! Your greenhouse tour will include a discussion of how the greenhouse provides for the environmental needs of growing plants.
Morphology. This step was started for you by the instructor two weeks ahead of class. Fill one pot with friable soil. Plant 10 bean seeds about 1 cm deep so that 1 cm of soil covers each seed. Place the pot on-line in the greenhouse.
Water Relations. Fill four plastic cups to within 1 cm of the rim with well-packed perlite (expanded lava). Label: 10x, 1x, 0.1x, and 0x with the marking pen. Also label each cup with your group identification. Water with the respective fertilizer solutions until the perlite floats. Plant ten radish seeds in each cup so that they are under-water but still visible. "1x" is the manufacturer's recommended concentration of fertilizer and "0x" is distilled water. Place the cups in the specially-designated area of the classroom where the plants will only be watered with distilled water.
Vegetative Propagation. Obtain two pots and fill to 1 cm below the ridge with friable potting soil. Firm and level the soil. Distribute one rounded teaspoon (at least 60!) mung bean seeds on the surface. Cover the seeds to 1 cm below the rim with vermiculite and place the pots on-line in the greenhouse.
Pruning. Obtain four pots and fill each with friable potting soil. Firm and level the soil to the ridge. Distribute ten tall pea seed over the soil and cover to 1 cm below the rim with friable soil. Place the pots on-line in the greenhouse.
Water Relations. Obtain three pots and fill, firm, and level to the ridge with friable potting soil. Label each pot with your group identification. Distribute ten radish seeds over the soil and cover with 1 cm of friable potting soil. Place the pots in a flat lacking drainage holes in the greenhouse as follows: Put one pot directly in the flat; label it "none". Place the second pot, labeled "twelve", inside twelve empty pots and put the stack in the flat. Place the third pot, labeled "eighteen", inside eighteen empty pots and put the stack in the flat. Place two water emitters in the "none" pot ONLY!
Light Relations. Obtain two pots and fill, firm, and level to the ridge with friable potting soil. Distribute ten dwarf pea seeds over the surface and cover with 1 cm of friable potting soil. Label the pots with your group identification; mark one "light" and the other "dark." Place the pot marked "light" on-line in the greenhouse and place the other pot under the inverted black wastebasket under the benches in the greenhouse.
Growth Hormones. Obtain eight pots and fill, firm, and level to the ridge with friable potting soil. Label each pot with your group identification. Label four pots with the words "pea stem growth - dwarf", label four pots with the words "pea stem growth - tall." Distribute 10 pea seeds and cover with 1 cm of friable potting soil; be careful to plant only dwarf seeds in the pots labeled dwarf and only tall seeds in the pots labeled tall. Place the pots on-line in the greenhouse.
Seed Germination. Obtain four pots and fill, firm, and level with friable potting soil to within 6 cm, 3 cm, 1.5 cm, and 0 cm of the ridge, respectively. Pat down the soil to make the surface firm and level. Label the pots by distance from ridge and group identification. Count out twenty (accurate!) lettuce seeds and 10 tall pea seeds and distribute them over the surface of the soil of each pot. Then carefully fill each pot only to the ridge with more friable potting soil. NOTE: the fourth pot will have NO additional soil and the seeds will be exposed on the surface! Place the pots on-line in the greenhouse.
|1.5 cm||3 cm||6 cm|
Obtain three pots and fill them with friable potting soil. Label the pots with your group identification and the word "Cuttings". Put a band of tape completely around the rim of each pot and divide this tape into four equal sectors. Label each sector as follows: "Untreated," "Hormodin #1," "Hormodin #2," "Hormodin #3." You will plant four cuttings in each pot.
Make four leaf-petiole cuttings of Kalanchoe (smooth leaves with scalloped margins) and Pepperomia (smooth leaves with smooth margins) and stem cuttings of Plectranthus (Cuban oregano or Swedish ivy). Treat one cutting with Hormodin #1 powder, one with Hormodin #2 powder, and one with Hormodin #3 powder. Leave the fourth cutting untreated. The treatments are made by dipping the petiole in plain water, shaking off the excess water, dipping in the powder, and shaking off the excess powder. Plant the four cuttings of a particular species, one per sector by treatment, in an upright position in friable potting soil in one of the pots. BE CAREFUL to put the cutting in the sector of the pot labeled with its treatment! Repeat for the other two genera.
Place the pots on-line in the greenhouse.
This page © Ross E. Koning 1994.
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